Tenneille E. Ludwig, PHD

Position title: Director, WiCell Stem Cell Bank

Email: tludwig@wicell.org

Phone: 608.291.6100 x1010

Organ System/Disease Focus:
Pluripotent Stem Cells
Aligned Research Focus:
Quality standards for hPSC research, hPSC regulatory concerns, RUO and cGMP hPSC culture and banking optimization


More information:
Research Description:

Tenneille Ludwig serves as the Director of the WiCell Stem Cell Bank overseeing the banking, distribution, and core services operations at WiCell. Dr. Ludwig holds a PhD in embryology and developmental biology with a minor in bioethics from the University of Wisconsin–Madison. Her post-doctoral work was in the laboratory of James Thomson on the optimization of cell culture conditions and resulted in the development of the first defined, feeder-independent culture system for human pluripotent stem cells (TeSR/mTeSR). In addition to her duties at WiCell, she is a member of the Stem Cell and Regenerative Medicine Center and serves on the ESCRO Committee at the University of Wisconsin–Madison. She works with multiple boards and organizations, collegiate training programs, international registries, and serves as a Steering Committee Member for the International Stem Cell Banking Forum (ISCBF), the International Stem Cell Initiative (ISCI) Genetics and Epigenetics Study Group, and the International Society for Stem Cell Research (ISSCR) Manufacturing, clinical Translation, and Industry Committee. Her primary focus remains collaborating within these groups to refine consensus standards for banking, characterization, and distribution of research and cGMP grade materials, and working with investigators to support the advancement of stem cell technologies through clinical trial.

Selected References:
  • Abranches E, Spyrou S, Ludwig T.  2020. “GMP Banking of Human Pluripotent Stem Cells: A US and UK Perspective.” Stem Cell Res. May;45. doi: 10.1016/j.scr.2020.101805
  • Ludwig TE, Kujak A, Rauti A, Andrzejewski S, Langbehn S, Mayfield J, Fuller J, Yashiro Y, Hara Y, Bhattacharyya A.  “20 Years of Human Pluripotent Stem Cell Research: It All Started with Five Lines.” Cell Stem Cell.  2018 Nov1;18 Volume 23, Issue 5, pp644-648.
  • Stacey G, Crook J, Hei D, Ludwig T.  2013. “Banking human induced pluripotent stem cells: Lessons learned from embryonic stem cells.”  Cell Stem Cell. Volume 3, Issue 4 pp385-388.  doi 10.1016/j.stem.2013.09.007
  • Ludwig TE, Bergendahl V, Levenstein ME, Yu J, Probasco MD, Thomson JA.  2006. “Feeder-independent culture of human embryonic stem cells.”  Nature Methods. Aug;3(8):637-46. Epub July 21.
  • Ludwig, TE, ME Levenstein, JM Jones, WT Berggren, ER Mitchen, JL Frane, LJ Crandall, CA Daigh, KR Conard, MS Piekarczyk, RA Llanas and JA Thomson.  2006. “Derivation of human embryonic stem cells in defined conditions.”  Nature Biotechnology. Feb;24(2):185-7. Epub 2006 Jan 1.