Melissa Harrison, PhD

Credentials: Biomolecular Chemistry

Position title: Associate Professor

Email: mharrison3@wisc.edu

Phone: 608-262-2382

Organ System/Disease Focus
Transcriptional regulation during development, Cancer
Aligned Research Focus
Cell differentiation, embryonic reprogramming, neural stem cells

Harrison Lab

Research Description

Pluripotent cells have the ability to become virtually any cell type, and the power to control this capacity could revolutionize both basic research and medicine. Advances in reprogramming of specified cell types back to a pluripotent state have generated enthusiasm for the potential to create and manipulate specific cell lineages in culture. While reprogramming has exciting possibilities, the process is inefficient, limiting the future therapeutic benefits. By contrast, reprogramming occurs rapidly and efficiently in the early embryo when fertilized eggs are remodeled to become the embryonic cells that will eventually generate the adult organism. Our group is focused on understanding the fundamental molecular mechanisms by which the embryonic genome is rapidly remodeled to create the pluripotent state. We have shown that this process relies on transcription factors with unique properties that allow them to define regulatory regions. Ongoing studies focus on the unique set of factors that can drive this reprogramming within the context of a developing metazoan and understanding how these proteins shape the genome to affect the dramatic changes in cell fate required for organismal development.

Selected References:
  1. McDaniel, S.L., Gibson, T.J., Schulz, K.N., Fernandez Garcia, M., Nevil, M.N., Jain, S.U., Lewis, P.W., Zaret, K.S., and M.M. Harrison. (2019) Continued activity of the pioneer factor Zelda is required to drive zygotic genome activation. Mol Cell. 74:185-195.
  2. Schulz, K.N. and M.M. Harrison. (2019) Mechanisms regulating zygotic genome activation. Nat. Rev. Genet. 20:221-234.
  3. Nevil, M., Gibson, T.J., Bartolutti, C., Iyengar, A., and Harrison, M.M. (2020) Establishment of chromatin accessibility by the conserved transcription factor Grainy head is developmentally regulated. Development. 147: doi: 10.1242/dev.185009
  4. Janssens, D.H., Hamm, D.C., Xiao, Q., Anhezini De Araujo, L., Siller, K.H., Siegrist, S.E., Harrison, M.M., and C.Y. Lee. (2017) A novel Hdac1/Rpd3-poised circuit balances continual self-renewal and rapid restriction of developmental potential during asymmetric stem cell division. Dev Cell 40: 367-380.