Emery H. Bresnick, PHD

Professor, Cell and Regenerative Biology

ehbresni@wisc.edu

608-265-6446

Organ System/Disease Focus:
Hematology and vascular biology
Aligned Research Focus:
Basic stem cell science; hematopoietic stem and progenitor cells

Emery Bresnick headshot

Pubmed

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Research Description:

We use multidisciplinary approaches to understand stem/progenitor cell function, blood cell development, and vascular biology. Such approaches include genomics, proteomics, chemical genetics, embryonic stem cell differentiation, computational analysis, and traditional methodologies.

In addition to elucidating biological principles, we aim to develop innovative therapeutic strategies based on targeting novel mechanisms.
Projects relevant to stem cell biology/regenerative medicine include:

Mechanisms of hematopoiesis:
We are analyzing the function/regulation of GATA factors that control hematopoietic stem cells (HSCs) and hematopoiesis. Transcriptional profiling and ChIPchip analysis have identified a cohort of novel GATA factor target genes, including genes encoding proteins that bear no obvious similarity to known proteins. Loss-of-function and gain-of-function studies are being conducted in mice, zebrafish, human adult stem/progenitors, and embryonic stem cell systems to discover new modes of controlling stem/progenitor cell function and to devise novel applications towards translational medicine. Defining novel hematopoietic mechanisms has enormous importance, as deviations from physiological programs yield leukemias, lymphomas, myelodysplasia, and additional blood disorders.

Vascular mechanisms and engineering::
We have developed a novel murine model characterized by severe hematopoietic stem/progenitor cell depletion and a severe vascular integrity defect. We are dissecting mechanisms underlying the establishment and maintenance of vascular integrity in normal and disease states. Understanding such mechanisms is essential for developing efficient approaches to promote the vascularization of engineered tissues, a limiting factor in regenerative medicine

Selected References:
  • Mehta C, Johnson KD, Gao X, Ong IM, Katsumura KR, McIver SC, Ranheim EA, Bresnick EH. Integrating Enhancer Mechanisms to Establish a Hierarchical Blood Development Program. Cell Rep. 2017 Sep 19;20(12):2966-2979. doi: 10.1016/j.celrep.2017.08.090. PubMed PMID: 28930689; PubMed Central PMCID: PMC5618720.
  • Hewitt KJ, Katsumura KR, Matson DR, Devadas P, Tanimura N, Hebert AS, Coon JJ, Kim JS, Dewey CN, Keles S, Hao S, Paulson RF, Bresnick EH. GATA Factor-Regulated Samd14 Enhancer Confers Red Blood Cell Regeneration and Survival in Severe Anemia. Dev Cell. 2017 Aug 7;42(3):213-225.e4. doi: 10.1016/j.devcel.2017.07.009. PubMed PMID: 28787589; PubMed Central PMCID: PMC5578808.
  • Katsumura KR, Bresnick EH; GATA Factor Mechanisms Group. The GATA factor revolution in hematology. Blood. 2017 Apr 13;129(15):2092-2102. doi: 10.1182/blood-2016-09-687871. Epub 2017 Feb 8. Review. PubMed PMID: 28179282; PubMed Central PMCID: PMC5391619.
  • Hewitt KJ, Kim DH, Devadas P, Prathibha R, Zuo C, Sanalkumar R, Johnson KD, Kang YA, Kim JS, Dewey CN, Keles S, Bresnick EH. Hematopoietic Signaling Mechanism Revealed from a Stem/Progenitor Cell Cistrome. Mol Cell. 2015 Jul 2;59(1):62-74. doi: 10.1016/j.molcel.2015.05.020. Epub 2015 Jun 11. PubMed PMID: 26073540; PubMed Central PMCID: PMC4499333.
  • Johnson KD, Kong G, Gao X, Chang YI, Hewitt KJ, Sanalkumar R, Prathibha R, Ranheim EA, Dewey CN, Zhang J, Bresnick EH. Cis-regulatory mechanisms governing stem and progenitor cell transitions. Sci Adv. 2015 Sep 4;1(8):e1500503. doi: 10.1126/sciadv.1500503. eCollection 2015 Sep. PubMed PMID: 26601269; PubMed Central PMCID: PMC4643771.