Marjorie Brand, PhD

Education

Ph.D., 2001, Louis Pasteur University (France), Molecular Biology (with Laszlo Tora)

Postdoctoral Research, Fred Hutchinson Cancer Research Center, Seattle, WA (with Mark Groudine)

Research

Hematopoietic Stem cells have the capacity to differentiate along multiple lineages potentially giving rise to
all cells present in the blood. This process is controlled by cell-specific and ubiquitously expressed transcription
factors and cofactors. Defects in the transcriptional regulatory network of these cells can lead to leukemia. The
major goal of the Brand laboratory is to decipher the molecular mechanism of hematopoietic stem cell
differentiation such that we can understand how deregulation of this process can contribute to disease
including leukemia and ß-thalassemia. Towards this goal, we are using a multi-disciplinary approach that
combines in vitro and in vivo techniques in both cell lines and primary human cells. These approaches include
relative and absolute quantitative proteomics (isotope tagged methods), single cell multi-omics (CITEseq,
TEAseq, sc-CUT&Tag, sc-RNAseq) and bioinformatics as well as patients-derived xenotransplantation models
of leukemia and leukemia murine models.

Representative Publications

• M.A. Gillespie*, C.G. Palii*, D. Sanchez-Taltavull*, P. Shannon, W.J.R. Longabaugh, D.J. Downes, K. Sivaraman, H.M. Espinoza, J.R. Hughes, N.D. Price, T.J. Perkins^**, J.A. Ranish^** & M. Brand^**.  Absolute Quantification of Transcription Factors Reveal Principles of Gene Regulation in Erythropoiesis. ^*co-first ^**co-senior Molecular Cell (2020)
• C.G. Palii^*, Q. Cheng^*, M.A. Gillespie, P. Shannon, M. Mazurczyk, G. Napolitani, N.D. Price, J.A. Ranish, E. Morrissey^**, D.R. Higgs^** & M. Brand^**.  Single cell proteomics reveal that quantitative changes in co-expressed lineage-specific transcription factors determine cell-fate. ^*co-first ^**co-senior Cell Stem Cell (2019)
• Benyoucef, C.G. Palii, C. Wang, C.J. Porter, A. Chu, F. Dai, V. Tremblay, P. Rakopoulos, K. Singh, S. Huang, F. Pflumio, J. Hebert, J-F. Couture, T.J. Perkins, K. Ge, F.J. Dilworth & M. Brand.  UTX inhibition as a selective epigenetic therapy against TAL1-driven T-cell Acute Lymphoblastic Leukemia .   Genes & Development, 30(5): 508-521, 2016
• C.G. Palii, B. Vulesevic, S. Fraineau, E. Pranckeviciene, A.J. Griffith, A. Chu, H. Faralli, Y. Li, B. McNeill, J. Sun, T.J. Perkins, F.J. Dilworth, C. Perez-Iratxeta, E.J. Suuronen, D.S. Allan & M. Brand.  Trichostatin A enhances vascular repair by injected human endothelial progenitors through increasing the expression of TAL1-dependent genes.   Cell Stem Cell, 14(5): 644-657, 2014