The Seminar is held each Tuesday during the semester from noon-1 p.m.
Title: Understanding Single Cell RNA Sequencing and It’s Utility in Stem Cell Research
Abstract: Single Cell RNA Sequencing (scRNAseq) gives stem cell researchers access to an unprecedented level of in depth phenotypic data on an individual cell basis. This has allowed stem cell researchers to gain a better understanding of the cell type heterogeneity that exists within organisms as well as allowing researchers to appreciate the heterogeneity of their in vitro stem cell culture systems. My work in the Slukvin Lab is focused upon improving generation of human and Non-Human Primate iPSC derived hematopoietic stem/progenitor cells (iHSPCs). To improve our iHSPCs , I have relied heavily upon discoveries derived from In Vivo scRNAseq publications. By merging these In Vivo scRNAseq discoveries with scRNAseq we’ve performed on our in Vitro derived iHSPCs, we have been able to better understand the phenotypic heterogeneity of our iHSPCs. These understandings are now driving our current work to improve our iHSPC differentiation. On Oct 15th, I will be discussing how we think about scRNAseq in the general context of hematopoietic stem cell research, as well as how we are utilizing scRNAseq for improving our human and Non-Human Primate derived iHSPCs.
This event is offered in a hybrid format:
- The in-person seminar is being held at the UWBC Auditorium.
- To join online, please click the zoom link below:
- https://uwmadison.zoom.us/j/96958883460
- *if you are asked for a passcode: 970506
- https://uwmadison.zoom.us/j/96958883460