Our Seminar Lab Series will be offered in hybrid format this Fall
- The in-person seminar is being held at the Wisconsin Institute for Discovery, DeLuca Forum.
- There will be sandwiches provided again this term!! Come join us!
—————– - To join online, please click the zoom link below:
- https://uwmadison.zoom.us/j/96958883460?pwd=bWVHbEdWdGxQdHMyOVkxTHV5UW9mdz09
- *if you are asked for a passcode: 936743
- https://uwmadison.zoom.us/j/96958883460?pwd=bWVHbEdWdGxQdHMyOVkxTHV5UW9mdz09
Talk Title:
“Autofluorescent imaging screens of hydrogels for iPSC-derived cardiomyocyte differentiation and maturation.”
Brief Abstract:
Induced pluripotent stem cells (iPSC) can be differentiated into cardiomyocytes (CM) for disease modeling and drug screening. Batch variability in differentiated cells frequently occurs due to heterogeneity in commercial culture substrates. We performed optical metabolic imaging (OMI) on synthetic hydrogels of varying stiffnesses and compositions and observed expected metabolic shifts as iPSCs differentiated. OMI revealed metabolic differences between cells cultured on heterogenous commercial substrates and synthetic hydrogels and can be used to monitor cell sensitivity to the microenvironment. These data demonstrate OMI is a powerful tool for identifying iPSC differentiation and maturation conditions, crucial in stem cell manufacturing.
Speaker:
Danielle Desa
Skalla & Murphy Lab
UW Madison