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Dr. Owen Tamplin, Assistant Professor, Department of Cell & Regenerative Biology, “Understanding the Blood Stem Cell Niche in Zebrafish using Correlative Light Sheet and Electron Microscopy”
April 7 @ 12:00 pm - 1:00 pm
The UW Stem Cell & Regenerative Medicine Center will continue to offer its weekly seminar, but we will transition to ONLINE only. We will be offering this live (synchronously), so you can participate in the Q & A session in real time.
To connect and join our live Tuesday Stem Cell Seminar Series (now online), please click on this public link: https://us.bbcollab.com/guest/ecfe2a1900ab4a6ca9bd6b20e4b7bb07
We do encourage you all to review the instructions, linked below, in order to orient yourselves to this environment. You are also welcome to jump into the room and test your ability to connect, including turning on your audio and video. Our center’s Administrator, Hollie, will be in the room starting at 10:30 AM CT and can offer assistance as time allows.
Guest Access Instructional Overview: https://go.wisc.edu/ftti09
If you have any questions, please contact Hollie: email@example.com
**STUDENTS: If you are a current student, an announcement has been posted to your Canvas course with instructions on how to access the course. IF you have issues getting into Canvas, please use guest link and let Hollie know via email.
Understanding the Blood Stem Cell Niche in Zebrafish using Correlative Light Sheet and Electron Microscopy
Dr. Owen Tamplin
Hematopoietic stem cells (HSCs) reside in a specialized microenvironment called the niche. Many different niche cell types support HSPCs, including endothelial and mesenchymal stromal cells. However, the precise ultrastructure of HSCs within their niche has been difficult to define. Here we show that single endogenous HSCs can be genetically tagged for live tracking by light microscopy, followed by serial block-face scanning electron microscopy (SBEM). Using the zebrafish larval kidney marrow niche as a model, we could follow a single fluorescently-labeled HSC by light sheet microscopy, then confirm its exact location in a 3D EM dataset by high contrast APEX2 peroxidase label. By integrating multiple imaging modalities, we could resolve the ultrastructure of single rare cells deep in live tissue, and define all contacts between an HSPC and its surrounding niche cell types.