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Dr. Randolph Ashton, “Bioengineering Early CNS Morphogenesis for a Scalable Neural Tube Defect Risk Assay”

March 31, 2020 @ 12:00 pm - 1:00 pm

The UW Stem Cell & Regenerative Medicine Center will continue to offer its weekly seminar, but we will transition to ONLINE only. We will be offering this live (synchronously), so you can participate in the Q & A session in real time.

To connect and join our live Tuesday Stem Cell Seminar Series (now online), please click on this public link: https://us.bbcollab.com/guest/ecfe2a1900ab4a6ca9bd6b20e4b7bb07

We do encourage you all to review the instructions, linked below, in order to orient yourselves to this environment. You are also welcome to jump into the room and test your ability to connect, including turning on your audio and video. Our center’s Administrator, Hollie, will be in the room starting at 10:30 AM CT and can offer assistance as time allows.

Guest Access Instructional Overview: https://go.wisc.edu/ftti09

If you have any questions, please contact Hollie: hjthomps@medicine.wisc.edu

**STUDENTS: If you are a current student, an announcement has been posted to your Canvas course with instructions on how to access the course. IF you have issues getting into Canvas, please use guest link and let Hollie know via email.

Bioengineering Early CNS Morphogenesis for a Scalable Neural Tube Defect Risk Assay

Dr. Randolph Ashton, Associate Professor, Biomedical Engineering & Wisconsin Institute for Discovery Associate Director, Stem Cell & Regenerative Medicine Center

Neural organoids derived from human pluripotent stem cells (hPSCs) are becoming powerful tools for investigating CNS development, physiology, and disease. However, the innate, spontaneous emergent properties of neurally differentiating hPSC aggregates, which make neural organoids possible, also limits their application due to inconsistencies in the organoid’s cellular composition and tissue cytoarchitecture. We hypothesized that this was caused by the absence of biophysical and biochemical cues normally present within the developing embryo. To reinstate such controls in vitro, we developed culture methods and platforms that enable facile spatiotemporal control of such cues to result in a standardization of early neural organoid morphogenesis. In this talk, our success in exerting biophysical control over microscale tissue morphology to standardize the derivation of singularly polarized, forebrain through spinal neuroepithelial tissues will be presented. This mimics the earliest stage of CNS morphogenesis, i.e. neural tube formation. Thus, the scaling of this culture platform to create a screen for quantitatively assessing a chemicals’ risk of causing congenital neural tube defects will also be discussed.



March 31, 2020
12:00 pm - 1:00 pm
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