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Gavin Knight, Postdoctoral Fellow, Biomedical Engineering, “Engineering Standardized Organotypic Neural Slice Cultures from Human Pluripotent Stem Cells”
December 11, 2018 @ 12:00 pm - 1:00 pm
Topic: Engineering Standardized Organotypic Neural Slice Cultures from Human Pluripotent Stem Cells
Abstract: Human pluripotent stem cell (hPSC)-derived neural organoids self-organize to recapitulate facets of morphogenesis unique to humans, partially alleviating some of the shortcomings and ethical concerns of developmental studies in animal models. However, neural organoids are large tissues that yield multiple regions of self-organization, known as neural rosettes. Further, neural rosettes emerge in random locations with indeterminate size and shape. Such inconsistency impedes reproducibility and reduces the utility of neural organoids. To standardize neural organoid morphogenesis we have engineered a micropatterned cell culture array substrate that controls the biophysical and biochemical aspects of hPSC neural differentiation. Here we present results demonstrating highly efficient and reproducible generation of neural tissues consisting of a single uniform neural rosette. Additionally, we will discuss the adaptation of this platform for generation of spinal neural organoids and it’s potential for developmental neurotoxicity screening.