Tongcheng Qian, Postdoctoral Fellow, Morgridge Institute for Research / Biomedical Engineering (Skala Lab), “Monitoring stem cell-derived cardiomyocyte maturation by optical metabolic imaging”
November 27 @ 12:00 pm - 1:00 pm
Topic: Monitoring stem cell-derived cardiomyocyte maturation by optical metabolic imaging
Abstract: Cardiovascular disease remains the leading cause of death in the world despite advances in treatment. Human pluripotent stem cells (hPSCs) can generate any cell lineage in vitro, including cardiomyocytes. hPSC-derived cardiomyocytes have immense potential to impact clinical care and fundamental research for cardiovascular disease. However, hPSC-derived cardiomyocytes exhibit a relatively immature phenotype. New technologies that can non-invasively quantify the maturation state in live hPSC-cardiomyocytes are needed to effectively optimize a mature phenotype. hPSC-derived cardiomyocytes undergo dramatic metabolic changes during maturation. Here, we monitor these metabolic changes that occur in hPSC-derived cardiomyocytes during extended time in culture by multiphoton fluorescence lifetime imaging (FLIM) of the metabolic co-enzymes NAD(P)H and FAD. Changes in hPSC-cardiomyocyte metabolism during maturation were non-invasively quantified at a single-cell level with the optical metabolic imaging (OMI) parametersOur preliminary results indicate that the lifetime of NAD(P)H increases and the redox ratio decreasess with maturation over a 100-day time-course in hPSC-derived cardiomyocytes. These label-free imaging technologies could be used to test strategies to optimize the maturation of hPSC-derived cardiomyocytes in vitro.