The Ludwig Laboratory
Faculty > Tenneille E. Ludwig
Tenneille E. Ludwig
Senior Scientist; Director of Distribution, Media Optimization and Core Services
Organ System/Disease Focus: Pluripotent Stem Cells
Aligned Research Focus: Culture and banking optimization
Research Description
Dr. Ludwig’s work in the laboratory of Dr. James Thomson (2001-2007) focused primarily on the optimization of human embryonic stem (ES) cell culture conditions, and resulted in the development of a defined, feeder-independent culture system for human ES cells (TeSR/mTeSR). Since moving to WiCell Research Institute, her primary research interest continues to be focused on improving human ES cell culture. Current projects include investigations into improving culture systems for pluripotent stem cells, focusing on feeder-independent, defined culture systems. Significant current research efforts include improving attachment and cloning efficiency in pluripotent (hES and iPS cells), enabling large-scale culture of human ES-cells, and developing alternate high throughput screening methods. She is also involved in optimization of banking practices for hES and iPS cells, to improve efficiency and reduce cost while maintaining quality and providing flexibility to the end user.
Selected References
Ludwig, TE and JA Thomson. 2007. Defined, feeder-independent medium for human embryonic stem cell culture. In: Current Protocols in Stem Cell Biology 1C.2.1-1C.2.16. John Wiley & Sons, Inc. Published online September 2007 in Wiley Interscience (www.interscience.wiley.com).
Ludwig, T.E. and J.A. Thomson. 2007. Defined culture media for human ES cells. In: Human ES Cell Culture Volume 6. Springer Publishing Corp.
Ludwig TE, Bergendahl V, Levenstein ME, Yu J, Probasco MD, Thomson JA. Feeder-independent culture of human embryonic stem cells. Nature Methods. 2006 Aug;3(8):637-46. Epub July 21.
Ludwig, TE, ME Levenstein, JM Jones, WT Berggren, ER Mitchen, JL Frane, LJ Crandall, CA Daigh, KR Conard, MS Piekarczyk, RA Llanas and JA Thomson. “Derivation of human embryonic stem cells in defined conditions.” Nature Biotechnology 2006 Feb;24(2):185-7. Epub 2006 Jan 1.
Ludwig, T. and J.A. Thomson. 2004. “Current progress in human embryonic stem cell research,” in Monitoring Stem Cell Research, A Report of the President's Council on Bioethics, 279-294.