The Hematti Laboratory
Faculty > Peiman Hematti
Peiman Hematti
Assistant Professor, Medicine, Hematology/Oncology Section
pxh@medicine.wisc.edu
Organ System/Disease Focus
Hematopoietic (blood) system; Treatment of hematological malignancies through hematopoietic stem cell transplantation
Aligned Research Focus
Bone marrow (hematopoietic and mesenchymal) stem cell biology
Research Description
Hematopoietic stem cells (HSCs) originating from the bone marrow are the best-known adult stem cells. Over the last few decades, HSC transplantation has saved the lives of thousands of patients with a variety of malignant and non-malignant hematological disorders. Bone marrow also contains another type of stem cell: mesenchymal stromal cells (MSCs) provide the niche for HSCs. Furthermore, MSCs have recently generated excitement in the field of regenerative medicine due to their multi-potential differentiation capacity and their immunomodulatory properties.
As a bone marrow transplant physician, I focus on the biology of bone marrow HSCs and MSCs. In my lab, we study how bone marrow MSCs exert their hematopoietic supportive activity and immunomodulatory functions. These basic research activities complement our clinical research activities: we are testing the potential of bone marrow stem cells in a variety of clinical trials.
We have also recently been able to derive MSCs from human and rhesus embryonic stem cells (ESCs) with characteristics very similar to bone marrow-derived MSCs. Our plan is to develop the rhesus macaque model as a relevant pre-clinical model to study the in vivo fate of ESC-derived MSCs.
Selected References
Hematti P, Sloand EM, Carvallo CA, Albert MR, Yee CL, Fuehrer MM, Blancato JK, Kearns WG, Barrett JA, Childs RW, Vogel JC, Dunbar CE. Absence of donor-derived keratinocyte stem cells in skin tissues cultured from patients after mobilized peripheral blood hematopoietic stem cell transplantation. Exp Hematol (8):943-9, 2002.
Hematti P, Sellers SE, Agricola BA, Metzger ME, Donahue RE, Dunbar CE. Retroviral transduction efficiency of G-CSF+SCF mobilized peripheral blood CD34+ cells is superior to G-CSF or G-CSF+Flt3-L mobilized cells in nonhuman primates. Blood 101:2199-2205., 2003.
Hematti P, Hong BK, Ferguson C, Adler R, Hanawa H, Sellers S, Holt IE, Eckfeldt CE, Sharma Y, Schmidt M, von Kalle C, Persons DA, Billings EM, Verfaillie CM, Nienhuis AW, Wolfsberg TG, Dunbar CE, Calmels B. Distinct genomic integration of MLV and SIV vectors in primate hematopoietic stem and progenitor cells. PLoS Biol 2(12):e423, 2004.
Larochelle A, Krouse A, Metzger M, Orlic D, Donahue RE, Fricker S, Bridger G, Dunbar CE, Hematti P. AMD3100 mobilizes hematopoietic stem cells with long-term repopulating capacity in non-human primates. Blood 107(9): 3772-3778, 2006.
Trivedi P, Hematti P. Simultaneous generation of CD34+ primitive hematopoietic cells and CD73+ mesenchymal stem cells from human embryonic stem cells cocultured with murine OP9 stromal cells. Exp Hematol 35(1):146-54, 2007.