The Golos Laboratory
Faculty > Thaddeus G. Golos
Thaddeus G. Golos
Professor, Obstetrics and Gynecology
golos@primate.wisc.edu
Organ System/Disease Focus
Implantation biology, placenta, embryo
Aligned Research Focus
Basic stem cell science, in vitro models of embryo implantation and development
Research Description
We use human and rhesus embryonic stem cells and rhesus embryos to study primate placental organogenesis. The first differentiation event in mammalian development is the specification of the trophectoderm lineage that will give rise to the placental trophoblasts, and the placenta is the first functional fetal organ.
Our central hypothesis is that the formation of the placenta relies on an interplay of extrinsic signals and intrinsic transcriptional regulatory pathways. We have developed systems in which to study placental morphogenesis by probing cell-cell and cell-matrix interactions with human ES cell co-culture systems and in vitro differentiation paradigms, and novel rhesus monkey embryo and ES cell in vitro and in vivo implantation models.
Selected References
Gerami-Naini B, Dovzhenko O, Durning M, Wegner F, Thomson J, Golos TG. Trophoblast differentiation in embryoid bodies derived from human embryonic stem cells. Endocrinology 145:1517-1524. 2004.
Liu YP, Burleigh DW, Durning M, Golos TG. The basic helix-loop-helix transcription factor E2-2 is the primary binding partner for Id2 in the human placenta. Cell. Mol. Endocrinol. 222:83-92. 2004.
Liu YP, Dovzhenko OV, Garthwaite MA, Dambaeva SV, Durning M, Pollastrini LM, Golos TG. Maintenance of Pluripotency in Human Embryonic Stem Cells Stably Over-Expressing Enhanced Green Fluorescent Protein. Stem Cells and Development. 13:636-645. 2004.